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KMID : 1094720150200010124
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 1 p.124 ~ p.130
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Experimental study and kinetic modeling of cometabolic degradation of phenol and p-nitrophenol by loofa-immobilized Ralstonia eutropha
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Maleki Mohammad
Motamedi Mahdi
Sedighi Mahsa
Zamir Seyed Morteza
Vahabzadeh Farzaneh
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Abstract
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In the present study, phenol-adapted cells of Ralstonia eutropha were used to degrade p-nitrophenol (PNP) in the presence of phenol. PNP at initial concentrations ranging from 5 to 15 mg/L was degraded almost completely by free cells of R. eutropha. The use of loofa-immobilized cells increased the complete removal of PNP up to 30 mg/L. Kinetic data for PNP biodegradation by immobilized cells of R. eutropha best fitted the Haldane model. The kinetic parameters were k s = 0.0006 (mg PNP/mg biomass.h), K s = 8.83 (mg/L) and K i = 30.77 (mg/L). The degradation pathways of PNP through the metabolites, 4-nitro-catechol (4-NC) and hydroquinone (HQ), were investigated using HPLC.
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KEYWORD
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cometabolism, kinetic modeling, loofa, phenol, p-nitrophenol
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